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Sample GSM170366 Query DataSets for GSM170366
Status Public on Dec 11, 2007
Title Day 28 control 6
Sample type RNA
 
Source name rat lung, day28, ctrl
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day28, ctrl
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 154.400000 P 0.000052
AFFX-BioB-3_st 5.700000 A 0.772364
AFFX-BioB-5_at 118.900000 P 0.003592
AFFX-BioB-5_st 40.800000 A 0.095667
AFFX-BioB-M_at 237.400000 P 0.000052
AFFX-BioB-M_st 12.400000 A 0.574038
AFFX-BioC-3_at 345.100000 P 0.000044
AFFX-BioC-3_st 1.100000 A 0.897835
AFFX-BioC-5_at 586.800000 P 0.000070
AFFX-BioC-5_st 3.200000 A 0.945787
AFFX-BioDn-3_at 1899.700000 P 0.000052
AFFX-BioDn-3_st 28.400000 A 0.205732
AFFX-BioDn-5_at 364.300000 P 0.000095
AFFX-BioDn-5_st 27.700000 A 0.216524
AFFX-CreX-3_at 5689.600000 P 0.000044
AFFX-CreX-3_st 3.100000 A 0.529760
AFFX-CreX-5_at 2910.300000 P 0.000044
AFFX-CreX-5_st 14.600000 A 0.275146
AFFX-DapX-3_at 1.500000 A 0.927300
AFFX-DapX-5_at 6.800000 A 0.382599

Total number of rows: 8799

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM170366.cel.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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