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Status
Public on Dec 11, 2007
Title
Day 42 control 2
Sample type
RNA
Source name
rat lung, day42, ctrl
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day42, ctrl
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
192.500000
P
0.000095
AFFX-BioB-3_st
12.200000
A
0.631562
AFFX-BioB-5_at
104.300000
P
0.001796
AFFX-BioB-5_st
27.500000
A
0.108979
AFFX-BioB-M_at
305.000000
P
0.000052
AFFX-BioB-M_st
14.800000
A
0.368438
AFFX-BioC-3_at
237.200000
P
0.000052
AFFX-BioC-3_st
6.400000
A
0.631562
AFFX-BioC-5_at
358.700000
P
0.000060
AFFX-BioC-5_st
1.700000
A
0.973889
AFFX-BioDn-3_at
969.600000
P
0.000060
AFFX-BioDn-3_st
58.800000
P
0.036569
AFFX-BioDn-5_at
290.900000
P
0.000127
AFFX-BioDn-5_st
28.300000
A
0.102165
AFFX-CreX-3_at
2760.900000
P
0.000044
AFFX-CreX-3_st
17.800000
A
0.195266
AFFX-CreX-5_at
1787.500000
P
0.000044
AFFX-CreX-5_st
20.400000
A
0.083592
AFFX-DapX-3_at
0.700000
A
0.988616
AFFX-DapX-5_at
3.900000
A
0.275146
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170374.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
