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Status
Public on Dec 11, 2007
Title
Day 56 smoke exposure 2
Sample type
RNA
Source name
rat lung, day56, smoke
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day56, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
155.400000
P
0.000044
AFFX-BioB-3_st
3.900000
A
0.772364
AFFX-BioB-5_at
120.900000
P
0.001593
AFFX-BioB-5_st
20.700000
A
0.108979
AFFX-BioB-M_at
280.200000
P
0.000052
AFFX-BioB-M_st
19.200000
A
0.185131
AFFX-BioC-3_at
242.200000
P
0.000052
AFFX-BioC-3_st
1.800000
A
0.529760
AFFX-BioC-5_at
337.900000
P
0.000070
AFFX-BioC-5_st
4.800000
A
0.891021
AFFX-BioDn-3_at
1338.700000
P
0.000052
AFFX-BioDn-3_st
43.000000
P
0.026111
AFFX-BioDn-5_at
318.700000
P
0.000070
AFFX-BioDn-5_st
30.900000
A
0.156732
AFFX-CreX-3_at
4981.800000
P
0.000044
AFFX-CreX-3_st
17.800000
A
0.216524
AFFX-CreX-5_at
3017.500000
P
0.000044
AFFX-CreX-5_st
17.800000
P
0.036569
AFFX-DapX-3_at
1.100000
A
0.976071
AFFX-DapX-5_at
0.900000
A
0.760956
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170398.cel.gz
1.8 Mb
(ftp) (http) CEL
Processed data included within Sample table
