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Status
Public on Dec 11, 2007
Title
Day 56 smoke exposure 5
Sample type
RNA
Source name
rat lung, day56, smoke
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day56, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
123.700000
P
0.000044
AFFX-BioB-3_st
4.300000
A
0.672921
AFFX-BioB-5_at
79.900000
P
0.001796
AFFX-BioB-5_st
9.900000
A
0.313723
AFFX-BioB-M_at
201.800000
P
0.000052
AFFX-BioB-M_st
8.500000
A
0.514890
AFFX-BioC-3_at
241.100000
P
0.000052
AFFX-BioC-3_st
1.700000
A
0.724854
AFFX-BioC-5_at
308.100000
P
0.000081
AFFX-BioC-5_st
1.800000
A
0.957038
AFFX-BioDn-3_at
1154.400000
P
0.000060
AFFX-BioDn-3_st
30.600000
M
0.058444
AFFX-BioDn-5_at
319.800000
P
0.000060
AFFX-BioDn-5_st
30.000000
A
0.123572
AFFX-CreX-3_at
2834.900000
P
0.000044
AFFX-CreX-3_st
30.900000
A
0.078002
AFFX-CreX-5_at
1869.600000
P
0.000044
AFFX-CreX-5_st
21.500000
P
0.020022
AFFX-DapX-3_at
0.600000
A
0.986189
AFFX-DapX-5_at
1.700000
A
0.470241
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170401.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
