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Status
Public on Dec 11, 2007
Title
Day 84 smoke exposure 3
Sample type
RNA
Source name
rat lung, day84, smoke
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day84, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
155.400000
P
0.000147
AFFX-BioB-3_st
4.000000
A
0.852061
AFFX-BioB-5_at
93.000000
P
0.005565
AFFX-BioB-5_st
27.800000
A
0.147939
AFFX-BioB-M_at
228.900000
P
0.000340
AFFX-BioB-M_st
18.300000
A
0.227636
AFFX-BioC-3_at
285.500000
P
0.000044
AFFX-BioC-3_st
1.400000
A
0.860518
AFFX-BioC-5_at
456.500000
P
0.000070
AFFX-BioC-5_st
3.200000
A
0.883887
AFFX-BioDn-3_at
1552.700000
P
0.000060
AFFX-BioDn-3_st
29.800000
A
0.275146
AFFX-BioDn-5_at
360.600000
P
0.000110
AFFX-BioDn-5_st
16.600000
A
0.313723
AFFX-CreX-3_at
5239.900000
P
0.000044
AFFX-CreX-3_st
6.100000
A
0.354453
AFFX-CreX-5_at
3467.600000
P
0.000044
AFFX-CreX-5_st
17.100000
A
0.095667
AFFX-DapX-3_at
1.600000
A
0.976071
AFFX-DapX-5_at
1.300000
A
0.544587
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170415.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
