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Sample GSM170434 Query DataSets for GSM170434
Status Public on Dec 11, 2007
Title Day 112 smoke exposure 6
Sample type RNA
 
Source name rat lung, day112, smoke
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day112, smoke
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 64.600000 M 0.046482
AFFX-BioB-3_st 4.900000 A 0.824672
AFFX-BioB-5_at 74.000000 P 0.010317
AFFX-BioB-5_st 31.600000 M 0.058444
AFFX-BioB-M_at 157.500000 P 0.000169
AFFX-BioB-M_st 17.000000 A 0.411380
AFFX-BioC-3_at 178.800000 P 0.000110
AFFX-BioC-3_st 3.400000 A 0.737173
AFFX-BioC-5_at 153.400000 P 0.001796
AFFX-BioC-5_st 4.700000 A 0.860518
AFFX-BioDn-3_at 1507.000000 P 0.000095
AFFX-BioDn-3_st 52.700000 A 0.078002
AFFX-BioDn-5_at 280.200000 P 0.000127
AFFX-BioDn-5_st 43.300000 A 0.095667
AFFX-CreX-3_at 3929.400000 P 0.000044
AFFX-CreX-3_st 22.600000 A 0.216524
AFFX-CreX-5_at 1956.500000 P 0.000052
AFFX-CreX-5_st 21.600000 A 0.108979
AFFX-DapX-3_at 1.500000 A 0.910522
AFFX-DapX-5_at 11.500000 M 0.058444

Total number of rows: 8799

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM170434.cel.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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