|
| Status |
Public on Dec 11, 2007 |
| Title |
Day 238 cessation control 4 |
| Sample type |
RNA |
| |
|
| Source name |
rat lung, day238, cess ctrl
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain : SD
Gender : Male
Tissue : Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
rat lung, day238, cess ctrl
|
| Data processing |
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
|
| |
|
| Submission date |
Feb 20, 2007 |
| Last update date |
Dec 11, 2007 |
| Contact name |
Christopher S Stevenson |
| Organization name |
Novartis Institutes for BioMedical Research
|
| Department |
Respiratory Diseases
|
| Street address |
Wimblehurst Road
|
| City |
Horsham |
| ZIP/Postal code |
RH12 5AB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE7079 |
Chronic rat exposure to cigarette smoke |
|
