|
| Status |
Public on Jul 26, 2007 |
| Title |
Abdominal Aortic Aneurysm2-M |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from human abdominal aortic aneurysm
|
| Organism |
Homo sapiens |
| Characteristics |
Ethnicity = Caucasian
Tissue = Abdominal Aortic Aneurysm
Sex = M
Age (years) = 63
Agilent BioAnalyzer 2100 RNA Integrity Number = 6.5
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Guanidinium isothiocyanate, cesium chloride
centrifugation (Sambrook et. al. Molecular Cloning: a
laboratory manual)
|
| Label |
biotin, streptavidin-Cy3
|
| Label protocol |
150 ng of total RNA was amplified and labeled using the
Illumina TotalPrep RNA Amplification Kit (Cat #IL1791),
manual version 0606
|
| |
|
| Hybridization protocol |
1.5 micrograms of labeled cRNA was used for hybridization, and
microarrays were washed and stained using standard Illumina
BeadChip 6 X 2 protocol, Doc. # 11176837 Rev. E
|
| Scan protocol |
Scanned on Illumina BeadStation 500GX using standard Illumina
protocol
|
| Description |
Abdominal aortic aneurysm obtained from surgery to correct.
|
| Data processing |
Raw signal values from Illumina BeadStudio Version 1.5.0.34
|
| |
|
| Submission date |
Feb 20, 2007 |
| Last update date |
Jul 26, 2007 |
| Contact name |
Gerard Tromp |
| E-mail(s) |
gerard.tromp@sanger.med.wayne.edu
|
| Phone |
313-577-8773
|
| Fax |
313-577-5218
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Center for Molecular Medicine and Genetics
|
| Street address |
3309 Scott Hall, 540 East Canfield Ave
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE7084 |
Expression data from the abdominal aorta and abdominal aortic aneurysm |
|
