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Status
Public on Feb 23, 2007
Title
Cerebral cortex, technical replicate 2 at Oregon National Primate Research Center
Sample type
RNA
Source name
rhesus macaques cerebral cortex
Organism
Macaca mulatta
Characteristics
Tissue: cerebral cortex
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Following fragmentation, the biotinylated cRNA were hybridized for 16 hr at 45C on GeneChip Rhesus Macaque Array
Scan protocol
GeneChips were scanned on Affymetrix scanner 3000
Description
Gene expression data from cerebral cortex
Data processing
The data were analyzed with Robust Multichip Analysis (RMA) using MAS background correction and quantile normalization within the center.
Submission date
Feb 21, 2007
Last update date
Feb 22, 2007
Contact name
Fenghai Duan
E-mail(s)
fduan@stat.brown.edu
Phone
401-863-2175
Organization name
Brown University
Department
Biostatistics
Street address
121 S. Main Street
City
Providence
State/province
RI
ZIP/Postal code
02912
Country
USA
Platform ID
GPL3535
Series (1)
GSE7094
Expression data from 5 rhesus tissues at 3 centers
Data table header descriptions
ID_REF
VALUE
RMA-calculated Signal intensity (with MAS Background correction)
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-3_at
6.946471812
P
0.000195157
AFFX-BioB-5_at
6.923692554
P
0.000662332
AFFX-BioB-M_at
7.235783982
P
7.01E-05
AFFX-BioC-3_at
8.493490574
P
5.17E-05
AFFX-BioC-5_at
8.248055574
P
6.02E-05
AFFX-BioDn-3_at
10.86549028
P
7.01E-05
AFFX-BioDn-5_at
9.493885082
P
4.43E-05
AFFX-CreX-3_at
11.97549184
P
4.43E-05
AFFX-CreX-5_at
11.65973581
P
5.17E-05
AFFX-DapX-3_at
4.12469501
A
0.969023668
AFFX-DapX-5_at
3.997340797
A
0.772363657
AFFX-DapX-M_at
4.727145589
A
0.749204408
AFFX-LysX-3_at
4.725405008
A
0.108978703
AFFX-LysX-5_at
3.982824509
A
0.603080402
AFFX-LysX-M_at
4.936971932
A
0.824671785
AFFX-Mmu-actin-3_s_at
12.62838768
P
0.001672877
AFFX-Mmu-actin-5_at
11.93641431
P
0.001672877
AFFX-Mmu-actin-M_at
12.07289162
P
0.001672877
AFFX-Mmu-actin-M_x_at
12.23663318
P
0.001672877
AFFX-Mmu-ef1a-3_x_at
13.05069549
P
0.001672877
Total number of rows: 52865 Table truncated, full table size 2442 Kbytes .Supplementary file
Size
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File type/resource
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CEL