|
| Status |
Public on Nov 07, 2007 |
| Title |
TS7 AdCreGFP Day 3 rep 2 Grp B |
| Sample type |
RNA |
| |
|
| Source name |
TS7_AdCreGFP Day 3 rep2
|
| Organism |
Mus musculus |
| Characteristics |
f_f; medium GM; AdenoCre; TS7_AdCreGFP Day 3 rep2
|
| Biomaterial provider |
In house cell line and cell culture
|
| Growth protocol |
TS cells were isolated from blastocysts and grown as previously described (Tanaka et al., 1998). Briefly, Growth medium (GM) was RPMI 1640 supplemented with 20% FBS, 1 mM sodium pyruvate, 100 μM b-mercaptoethanol, 100 U/ml penicillin, 100μg/ml streptomycin, 2 mM L-glutamine, 25 ng/ml human recombinant FGF4 (Sigma), 1 μg/ml heparin, and 70% EFCM. TS cell differentiation was induced by withdrawal of FGF4 and EFCM after trypsinization and replating at appropriate density. For TS colony formation assay, optimally growing TS cells were trypsinized and replated at 500-2000 cells per 6-cm dish onto feeder cells, and fed with fresh GM every 2-3 days for 1-2 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina TotalPrep RNA amplification kit (Ambion Catalog#IL1791)
|
| Label |
Streptavidin Cy3
|
| Label protocol |
As described in the illumina Mouse-6 chip processing protocol. Briefly, generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
As recommended by illumina.
|
| Scan protocol |
As suggested by Illumina using the Illumina scanner.
|
| Description |
#2 biological replicate from two for the transfected TS7_AdCreGFP Day 3 rep1 cell line on day 3 in GM media.
|
| Data processing |
None: raw data from Beadstudio
|
| |
|
| Submission date |
Feb 21, 2007 |
| Last update date |
Nov 07, 2007 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE7238 |
Ets2 is required for trophoblast stem cell self renewal |
|
