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Sample GSM1709430 Query DataSets for GSM1709430
Status Public on Jan 22, 2016
Title 118_E_2_S
Sample type RNA
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
VALUE RMA summarized log2 values

Data table
11715100_at 5.421323479
11715101_s_at 4.850115735
11715102_x_at 4.386139996
11715103_x_at 5.522115891
11715104_s_at 3.862764444
11715105_at 4.295758683
11715106_x_at 4.671989586
11715107_s_at 4.588239814
11715108_x_at 5.250154395
11715109_at 4.188131271
11715110_at 4.474097072
11715111_s_at 4.532041241
11715112_at 5.853417573
11715113_x_at 5.38647465
11715114_x_at 5.320737773
11715115_s_at 3.730210224
11715116_s_at 4.026339978
11715117_x_at 3.652259709
11715118_s_at 5.788776088
11715119_s_at 7.448268282

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM1709430_118_alvarAgusti140413_Hg_U219_96_H07.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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