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Sample GSM1709443 Query DataSets for GSM1709443
Status Public on Jan 22, 2016
Title 32_NE_2_N
Sample type RNA
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: NO
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
VALUE RMA summarized log2 values

Data table
11715100_at 4.762044071
11715101_s_at 4.563801937
11715102_x_at 4.495984118
11715103_x_at 5.825312935
11715104_s_at 3.954524832
11715105_at 4.181753603
11715106_x_at 4.478407897
11715107_s_at 4.216044143
11715108_x_at 4.750162549
11715109_at 4.355054588
11715110_at 4.547952656
11715111_s_at 4.983257484
11715112_at 7.73139162
11715113_x_at 4.991078076
11715114_x_at 5.124956257
11715115_s_at 3.420402971
11715116_s_at 3.881891429
11715117_x_at 3.41330434
11715118_s_at 5.394495265
11715119_s_at 10.25382822

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM1709443_32_alvarAgusti140413_Hg_U219_96_C02.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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