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Sample GSM1709451 Query DataSets for GSM1709451
Status Public on Jan 22, 2016
Title 47_E_2_L
Sample type RNA
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
VALUE RMA summarized log2 values

Data table
11715100_at 4.769868068
11715101_s_at 4.756671611
11715102_x_at 4.704441583
11715103_x_at 5.20187905
11715104_s_at 4.107272373
11715105_at 4.362114415
11715106_x_at 4.706758492
11715107_s_at 4.236012069
11715108_x_at 4.924490414
11715109_at 4.12482062
11715110_at 4.507648307
11715111_s_at 4.829432044
11715112_at 4.40379881
11715113_x_at 5.019476337
11715114_x_at 4.815480634
11715115_s_at 3.529404719
11715116_s_at 4.157214537
11715117_x_at 3.586264225
11715118_s_at 5.600275726
11715119_s_at 7.700241076

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM1709451_47_alvarAgusti140413_Hg_U219_96_D03.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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