|
| Status |
Public on Jan 22, 2016 |
| Title |
52_E_2_L |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue from COPD patient
|
| Organism |
Homo sapiens |
| Characteristics |
emphysema: YES
gold stage: 2
tissue: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
|
| Label |
biotin
|
| Label protocol |
cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
|
| |
|
| Hybridization protocol |
Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
|
| Scan protocol |
GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
|
| Description |
former smoker
|
| Data processing |
Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
|
| |
|
| Submission date |
Jun 12, 2015 |
| Last update date |
Jan 22, 2016 |
| Contact name |
Maria Rosa Faner |
| E-mail(s) |
rfaner@clinic.ub.es
|
| Organization name |
Fundació Privada Clinic
|
| Department |
Cellex
|
| Lab |
606
|
| Street address |
C/Casanova 143, planta 2A,
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
|
