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Sample GSM1709458 Query DataSets for GSM1709458
Status Public on Jan 22, 2016
Title 53_E_2_L
Sample type RNA
 
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
 
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
 
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
E-mail(s) rfaner@clinic.ub.es
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
ID_REF
VALUE RMA summarized log2 values

Data table
ID_REF VALUE
11715100_at 4.764326748
11715101_s_at 4.789407642
11715102_x_at 4.410909366
11715103_x_at 5.262507887
11715104_s_at 3.959434316
11715105_at 4.109475852
11715106_x_at 4.892454341
11715107_s_at 4.88202125
11715108_x_at 5.193671412
11715109_at 4.124862362
11715110_at 4.415919369
11715111_s_at 5.015927687
11715112_at 5.25370924
11715113_x_at 5.428013329
11715114_x_at 5.765512859
11715115_s_at 3.582437868
11715116_s_at 4.040039364
11715117_x_at 3.63060482
11715118_s_at 5.509639376
11715119_s_at 7.652988531

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM1709458_53_alvarAgusti140413_Hg_U219_96_D09.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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