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Sample GSM1709460 Query DataSets for GSM1709460
Status Public on Jan 22, 2016
Title 55_E_2_S
Sample type RNA
 
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
 
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
 
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
E-mail(s) rfaner@clinic.ub.es
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
ID_REF
VALUE RMA summarized log2 values

Data table
ID_REF VALUE
11715100_at 4.93209024
11715101_s_at 4.836517106
11715102_x_at 4.778749607
11715103_x_at 5.665331775
11715104_s_at 3.940076876
11715105_at 4.11171526
11715106_x_at 4.888931915
11715107_s_at 4.666051998
11715108_x_at 5.059621784
11715109_at 4.35085221
11715110_at 4.451454197
11715111_s_at 4.74612916
11715112_at 4.567556093
11715113_x_at 5.439916194
11715114_x_at 5.962408668
11715115_s_at 3.691800003
11715116_s_at 4.039101896
11715117_x_at 3.430416035
11715118_s_at 5.326311704
11715119_s_at 7.149958949

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM1709460_55_alvarAgusti140413_Hg_U219_96_D11.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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