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Sample GSM1709461 Query DataSets for GSM1709461
Status Public on Jan 22, 2016
Title 56_E_2_L
Sample type RNA
 
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
 
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
 
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
E-mail(s) rfaner@clinic.ub.es
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
ID_REF
VALUE RMA summarized log2 values

Data table
ID_REF VALUE
11715100_at 4.66291546
11715101_s_at 5.075278159
11715102_x_at 4.713073383
11715103_x_at 5.17964448
11715104_s_at 4.016322739
11715105_at 4.121885793
11715106_x_at 4.727311329
11715107_s_at 4.422235679
11715108_x_at 4.93780802
11715109_at 4.241521682
11715110_at 4.435381785
11715111_s_at 4.906964391
11715112_at 4.619423466
11715113_x_at 5.150454789
11715114_x_at 5.198048658
11715115_s_at 3.491018486
11715116_s_at 3.791472054
11715117_x_at 3.598305395
11715118_s_at 5.471794895
11715119_s_at 10.08608606

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM1709461_56_alvarAgusti140413_Hg_U219_96_D12.CEL.gz 2.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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