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Sample GSM1709463 Query DataSets for GSM1709463
Status Public on Jan 22, 2016
Title 58_E_2_L
Sample type RNA
 
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
 
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
 
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
E-mail(s) rfaner@clinic.ub.es
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
ID_REF
VALUE RMA summarized log2 values

Data table
ID_REF VALUE
11715100_at 4.868863609
11715101_s_at 4.521700827
11715102_x_at 4.567071052
11715103_x_at 5.213031365
11715104_s_at 3.887486742
11715105_at 4.092580055
11715106_x_at 5.090197596
11715107_s_at 4.349785546
11715108_x_at 4.927352532
11715109_at 4.091931003
11715110_at 4.527515703
11715111_s_at 4.800057183
11715112_at 7.109482073
11715113_x_at 5.257565618
11715114_x_at 5.10144404
11715115_s_at 3.649810025
11715116_s_at 3.640355348
11715117_x_at 3.350181263
11715118_s_at 5.352718598
11715119_s_at 6.673946793

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM1709463_58_alvarAgusti140413_Hg_U219_96_E02.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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