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Sample GSM1709472 Query DataSets for GSM1709472
Status Public on Jan 22, 2016
Title 68_E_2_L
Sample type RNA
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
VALUE RMA summarized log2 values

Data table
11715100_at 4.677007116
11715101_s_at 5.033621732
11715102_x_at 4.233327492
11715103_x_at 5.350596622
11715104_s_at 4.133274079
11715105_at 4.099741133
11715106_x_at 5.054861509
11715107_s_at 4.3569679
11715108_x_at 5.305657698
11715109_at 4.329324414
11715110_at 4.423721227
11715111_s_at 4.857470884
11715112_at 5.731016531
11715113_x_at 5.388693824
11715114_x_at 5.566531159
11715115_s_at 3.571809533
11715116_s_at 4.025917634
11715117_x_at 3.524936173
11715118_s_at 5.453522086
11715119_s_at 7.492591401

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM1709472_68_alvarAgusti140413_Hg_U219_96_E12.CEL.gz 2.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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