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Sample GSM1709476 Query DataSets for GSM1709476
Status Public on Jan 22, 2016
Title 75_NE_2_L
Sample type RNA
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: NO
gold stage: 2
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
VALUE RMA summarized log2 values

Data table
11715100_at 5.128425602
11715101_s_at 4.947744874
11715102_x_at 4.514935477
11715103_x_at 5.771096743
11715104_s_at 3.905340969
11715105_at 4.276451519
11715106_x_at 4.827630563
11715107_s_at 4.336422585
11715108_x_at 5.248682342
11715109_at 4.286443155
11715110_at 4.568097541
11715111_s_at 4.740022106
11715112_at 7.69774464
11715113_x_at 5.386424622
11715114_x_at 5.547919453
11715115_s_at 3.611380422
11715116_s_at 3.802003276
11715117_x_at 3.855329773
11715118_s_at 5.70678849
11715119_s_at 7.273289878

Total number of rows: 49386

Table truncated, full table size 1225 Kbytes.

Supplementary file Size Download File type/resource
GSM1709476_75_alvarAgusti140413_Hg_U219_96_F04.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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