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Sample GSM1709487 Query DataSets for GSM1709487
Status Public on Jan 22, 2016
Title T-42_E_4_S
Sample type RNA
 
Source name Lung tissue from COPD patient
Organism Homo sapiens
Characteristics emphysema: YES
gold stage: 4
tissue: Lung
Extracted molecule total RNA
Extraction protocol RNA later presserved tissue, RNA extracted with miRNeasy Mini Kit, Quigen
Label biotin
Label protocol cRNA amplification and biotin labeling was synthesized from 150 ng total RNA by means of cDNA synthesis followed by an in vitro transcription reaction in the presence of T7 RNA Polymerase and biotinylated nucleotide analog/ribonucleotide mix (3'IVT Express Kit, Affymetrix Inc, Santa Clara, CA)
Biotin-labeled cRNA was cleanup and quantified with magnetic beads and subsequently fragmented.
 
Hybridization protocol Hybridization was performed with GeneChip® Hybridization Wash and Stain Kit (Affymetrix Inc) according to manufactures instructions
Scan protocol GeneChips were scanned using the GeneChip®Scanner 3000 7G (Affymetrix Inc).
Description former smoker
Data processing Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
 
Submission date Jun 12, 2015
Last update date Jan 22, 2016
Contact name Maria Rosa Faner
E-mail(s) rfaner@clinic.ub.es
Organization name Fundaci├│ Privada Clinic
Department Cellex
Lab 606
Street address C/Casanova 143, planta 2A,
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13667
Series (1)
GSE69818 COPD lung tissue expression

Data table header descriptions
ID_REF
VALUE RMA summarized log2 values

Data table
ID_REF VALUE
11715100_at 4.882718774
11715101_s_at 4.727057104
11715102_x_at 4.673412757
11715103_x_at 5.740455563
11715104_s_at 3.90974573
11715105_at 4.093130603
11715106_x_at 4.494340705
11715107_s_at 4.263349605
11715108_x_at 5.164466782
11715109_at 4.369812445
11715110_at 4.235183857
11715111_s_at 4.623612061
11715112_at 4.310365665
11715113_x_at 5.555460618
11715114_x_at 5.668306382
11715115_s_at 3.489164775
11715116_s_at 4.001806818
11715117_x_at 3.505400803
11715118_s_at 5.703869459
11715119_s_at 8.833439739

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM1709487_T-42_alvarAgusti140413_Hg_U219_96_G10.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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