|
| Status |
Public on Jun 16, 2015 |
| Title |
D37 |
| Sample type |
RNA |
| |
|
| Source name |
retinal pigmented epithelium (RPE)
|
| Organism |
Mus musculus |
| Characteristics |
date: 2012-03-07
tissue: retinal pigmented epithelium (RPE)
treatment: The Dct-Cre mice where crossed with Dicer1loxP to establish to establish Dicer1loxP/loxP;Dct-Cre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy kit (Qiagen) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
|
| Scan protocol |
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
|
| Description |
gene expression data of Dicer1 deficient RPE
Dicer1 flox/+ Dct cre mice where mated with Dicer1folx/flox mice. Pups where sacrifced at P5 and enucleated. Eyes where the dissected isolating the RPE.
|
| Data processing |
Probe-set analyses was performed. RMA normalization and summarization by mean, all were done by Partek Genomics suite (version 6.6)
Log2 expression intensities after normalization and summarization, as implicated in the data processing raw.
|
| |
|
| Submission date |
Jun 15, 2015 |
| Last update date |
Jun 16, 2015 |
| Contact name |
Metsada Pasmanik-Chor |
| E-mail(s) |
metsada@tauex.tau.ac.il
|
| Organization name |
Tel Aviv University
|
| Department |
Biology
|
| Lab |
Bioinformatics Unit
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL10740 |
| Series (2) |
| GSE69881 |
MicroRNAs of the RPE are essential for RPE differentiation and photoreceptor maturation (mRNA) |
| GSE69883 |
MicroRNAs of the RPE are essential for RPE differentiation and photoreceptor maturation |
|
