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Sample GSM171880 Query DataSets for GSM171880
Status Public on Mar 07, 2007
Title H524(A)
Sample type RNA
 
Source name lung cancer cell line
Organism Homo sapiens
Characteristics Histology: SCLC
Age: 63
Sex: M
Race: caucasian
Biomaterial provider Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center
Growth protocol RPMI 5% BFS
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit including DNase1 treatment
Label biotin
Label protocol Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA
 
Hybridization protocol Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description lung cancer cell line
Data processing Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250
 
Submission date Feb 26, 2007
Last update date Sep 01, 2016
Contact name Luc Girard
E-mail(s) Luc.Girard@UTSouthwestern.edu
Organization name UT Southwestern Medical Center
Department Hamon Center for Therapeutic Oncology Research
Lab NB8.114A
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390-8593
Country USA
 
Platform ID GPL96
Series (1)
GSE4824 Analysis of lung cancer cell lines
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF Affymetrix IDs
Stat Pairs Number of probe pairs
Stat Pairs Used Number of probe pairs (unmasked)
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF Stat Pairs Stat Pairs Used VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 20 20 593.0 P 0.001410
AFFX-BioB-M_at 20 20 1040.0 P 0.000169
AFFX-BioB-3_at 20 20 589.4 P 0.000169
AFFX-BioC-5_at 20 20 1227.3 P 0.000169
AFFX-BioC-3_at 20 20 1067.5 P 0.000070
AFFX-BioDn-5_at 20 20 872.7 P 0.000095
AFFX-BioDn-3_at 20 20 6956.0 P 0.000081
AFFX-CreX-5_at 20 20 12161.6 P 0.000044
AFFX-CreX-3_at 20 20 17674.2 P 0.000044
AFFX-DapX-5_at 20 20 30.0 A 0.455413
AFFX-DapX-M_at 20 20 41.4 A 0.287743
AFFX-DapX-3_at 20 20 5.7 A 0.916408
AFFX-LysX-5_at 20 20 35.3 M 0.062929
AFFX-LysX-M_at 20 20 6.5 A 0.834139
AFFX-LysX-3_at 20 20 7.6 A 0.659339
AFFX-PheX-5_at 20 20 3.7 A 0.891021
AFFX-PheX-M_at 20 20 2.9 A 0.953518
AFFX-PheX-3_at 20 20 17.4 A 0.645547
AFFX-ThrX-5_at 20 20 9.1 A 0.749204
AFFX-ThrX-M_at 20 20 17.6 A 0.514890

Total number of rows: 22283

Table truncated, full table size 728 Kbytes.




Supplementary file Size Download File type/resource
GSM171880.CEL.gz 3.4 Mb (ftp)(http) CEL

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