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Sample GSM1720835 Query DataSets for GSM1720835
Status Public on Jun 25, 2015
Title quad-control-3
Sample type RNA
 
Source name quadriceps muscle
Organism Mus musculus
Characteristics tissue: quadriceps
genotype: control
Sex: male
age: 41 days old
Treatment protocol Mice are homozygous for the conditional nebulin allele and are hemizygous for the muscle-specific MCK-Cre transgene (cKO) or lack the MCK-Cre transgene (Control).
Growth protocol Mice are on a mixed C57BL/6J and 1219S6/SvEvTac background. Mice are grown in a pathogen-free barrier facility with 12hr light:12hr dark cycle and are provided water and Teklad Global Rodent Diet (2919) ad libitum.
Extracted molecule total RNA
Extraction protocol Tissues were stored in Invitrogen RNAlater following manufacturer's protocol. Total RNA was isolated using Qiagen RNeasy fibrous tissue kit following the manufacturer's protocol.
Label biotin
Label protocol Samples were biotin-labelled using the Affymetrix GeneChip WT Plus reagant per manufacturer's protocol.
 
Hybridization protocol Samples were hybridized at 45C 17hours using the Affymetrix GeneChip Hybridization Buffers in with the Affymetrix Hyb Oven 645 and Fluidics Station 450.
Scan protocol The Affymetrix Scanner 3000 7G was used to scan the GeneChips using Affymetrix GeneChip Console.
Description normal quadriceps
Data processing Affymetrix Expression Console used RMA default settings and MoGene-1_0-st-v1.na34.mm10.transcript.csv annotation file. Affymetrix Transcript Analysis Console was used with One-Way Between-Subject ANOVA (Unpaired) and default filter criteria: Fold Change (linear) < -2 or Fold Change (linear) > 2 and ANOVA p-value (Condition pair) < 0.05.
 
Submission date Jun 24, 2015
Last update date Jun 25, 2015
Contact name Henk L Granzier
E-mail(s) granzier@email.arizona.edu
Organization name University of Arizona
Street address 1656 E Mabel St.
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
 
Platform ID GPL6246
Series (1)
GSE70213 The effect of Nebulin-Deficiency on Skeletal Muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10344614 41.02539
10344616 3.015051
10344620 6.285958
10344622 43.46384
10344624 533.7327
10344633 387.2067
10344637 78.44324
10344653 5.720942
10344658 245.1436
10344674 5.91184
10344679 15.59568
10344707 489.8102
10344713 238.3944
10344715 4.9725
10344717 8.873013
10344719 32.39105
10344721 2.931442
10344723 27.18478
10344725 236.5083
10344741 511.8004

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1720835_ControlQuad3.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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