|
| Status |
Public on Jun 25, 2015 |
| Title |
quad-cKO-2 |
| Sample type |
RNA |
| |
|
| Source name |
quadriceps muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: quadriceps
genotype: nebulin KO
Sex: male
age: 41 days old
|
| Treatment protocol |
Mice are homozygous for the conditional nebulin allele and are hemizygous for the muscle-specific MCK-Cre transgene (cKO) or lack the MCK-Cre transgene (Control).
|
| Growth protocol |
Mice are on a mixed C57BL/6J and 1219S6/SvEvTac background. Mice are grown in a pathogen-free barrier facility with 12hr light:12hr dark cycle and are provided water and Teklad Global Rodent Diet (2919) ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were stored in Invitrogen RNAlater following manufacturer's protocol. Total RNA was isolated using Qiagen RNeasy fibrous tissue kit following the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were biotin-labelled using the Affymetrix GeneChip WT Plus reagant per manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized at 45C 17hours using the Affymetrix GeneChip Hybridization Buffers in with the Affymetrix Hyb Oven 645 and Fluidics Station 450.
|
| Scan protocol |
The Affymetrix Scanner 3000 7G was used to scan the GeneChips using Affymetrix GeneChip Console.
|
| Description |
nebulin-deficient quadriceps
|
| Data processing |
Affymetrix Expression Console used RMA default settings and MoGene-1_0-st-v1.na34.mm10.transcript.csv annotation file. Affymetrix Transcript Analysis Console was used with One-Way Between-Subject ANOVA (Unpaired) and default filter criteria: Fold Change (linear) < -2 or Fold Change (linear) > 2 and ANOVA p-value (Condition pair) < 0.05.
|
| |
|
| Submission date |
Jun 24, 2015 |
| Last update date |
Jun 25, 2015 |
| Contact name |
Henk L Granzier |
| E-mail(s) |
granzier@email.arizona.edu
|
| Organization name |
University of Arizona
|
| Street address |
1656 E Mabel St.
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85724 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE70213 |
The effect of Nebulin-Deficiency on Skeletal Muscle |
|
