|
| Status |
Public on Jul 30, 2007 |
| Title |
B6By.C.132.54 strain, brain hemisphere, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Brain hemisphere of adult male mouse, cerebellum not included
|
| Organism |
Mus musculus |
| Characteristics |
Inbred strain: B6By.C.132.54; Gender: male; Age: 11-week-old (+/- 1 week)
|
| Treatment protocol |
Animals were killed by cervical dislocation, brains were quickly removed, hemisectioned, and alternate left or right hemispheres were collected in RNA-Later. After processing each hemisphere separately, 10 hemispheres were pooled for one high-density oligonucleotide microarray
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Fragmented cRNA probes were prepared from 7 µg of total RNA according to the protocol of Affymetrix using Superscript Choice System (Invitrogen) and a RNA Transcript Labeling Kit (Enzo, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChipR Scanner
|
| Description |
Gene expression data from brain hemispheres of experimentally naive male mice
|
| Data processing |
We used .CEL files produced by GCOS for probe (cell) level Robust Multi-Array Average (RMA)-based normalization of data as implemented in GeneTraffic ver. 3.0
|
| |
|
| Submission date |
Feb 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Csaba Vadasz |
| E-mail(s) |
vadasz@nki.rfmh.org
|
| Organization name |
New York University NKI
|
| Department |
Center for Neurobehavior Genetics
|
| Street address |
140 Old Orangeburg Road
|
| City |
Orangeburg |
| State/province |
NY |
| ZIP/Postal code |
10962 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7155 |
Expression data from adult laboratory mouse brain hemispheres |
|
| Relations |
| Reanalyzed by |
GSE119085 |
