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Sample GSM17243 Query DataSets for GSM17243
Status Public on Oct 26, 2004
Title Mouse liver (MoLi.231x)
Sample type MPSS
Source name Mouse liver total RNA
Organism Mus musculus
Extracted molecule total RNA
Description Strain: C57BL6
Mouse liver total RNA (MoLi.231x) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Library MoLi.231x.sig21
Cell type Mouse liver
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MoLi.231x_sig21.4791T.b-20 12/11/2003 404736 4303
MoLi.231x_sig21.4791T.a-20 12/10/2003 401571 4990
MoLi.231x_sig21.4791W.b-20 12/9/2003 493005 6186
MoLi.231x_sig21.4791W.a-20 12/9/2003 533054 6468
Run group:
Total Beads successfully sequenced - 1832366
Processed Signatures - 7105
Transcripts 3'-most (Canonical) Signatures - 4864
Lot batch =
Submission date Feb 26, 2004
Last update date May 28, 2005
Contact name Daixing Zhou
Phone 510-670-9441
Fax 510-670-9302
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
Platform ID GPL1010
Series (1)
GSE1067 MPSS analysis of mouse BLK CL.4 and liver cells

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global

Total number of rows: 4864

Table truncated, full table size 143 Kbytes.

Supplementary file Size Download File type/resource
GSM17243.raw.txt.gz 77.6 Kb (ftp)(http) TXT

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