|
| Status |
Public on Mar 08, 2007 |
| Title |
KO mouse 1_heart_basal |
| Sample type |
RNA |
| |
|
| Source name |
KO mouse heart under basal conditions
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6J
Gender: male
Age: 2-3 months
Tissue: heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Fibrous Tissue Midi kit (mortar and pestle)
|
| Label |
biotin
|
| Label protocol |
Refer to the protocols on the McGill University and Genome Quebec Innovation Centre website (http://www.genomequebec.mcgill.ca/services/microarray.php?page=tech).
|
| |
|
| Hybridization protocol |
Refer to the protocols on the McGill University and Genome Quebec Innovation Centre website (http://www.genomequebec.mcgill.ca/services/microarray.php?page=tech).
|
| Scan protocol |
Refer to the protocols on the McGill University and Genome Quebec Innovation Centre website (http://www.genomequebec.mcgill.ca/services/microarray.php?page=tech). An Affymetrix GeneChIP 3000 scanner was used.
|
| Description |
All hybridization were performed at the McGill University and Genome Quebec Innovation Centre. Affymetrix array technology involves the in-situ synthesis of hundreds of thousands of distinct oligonucleotide sequences onto a glass array using photolithography and combinatorial chemistry. Each 25-mer oligonucleotide is represented by millions of copies in a specific area. Each transcriptional sequence is spanned by 11-20 pairs of oligonucleotide probes randomly spaced throughout the array. All sequences designed on the array are selected from GenBank, dbEST and RefSeq. Target RNA is reverse transcribed into cDNA and in-vitro transcription is performed to generate biotin-labeled cRNA for subsequent hybridization. Hybridized target cRNA is stained with streptavidin phycoerythrin and arrays are scanned using a GeneArray Scanner at an excitation wavelength of 488nm. Light emissions at 570nm are proportional to the bound target at each oligonucleotides position on the GeneChip® array.
|
| Data processing |
The signal intensities shown in the VALUE column are a measure of abundance of transcript and calculated from combined, background adjusted perfect match and mismatched values of the probe set. The raw data is in MAS 5.0 format.
|
| |
|
| Submission date |
Mar 05, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Vincent Giguère |
| E-mail(s) |
vincent.giguere@mcgill.ca
|
| Phone |
514-398-5899
|
| Organization name |
McGill University
|
| Department |
Goodman Cancer Centre
|
| Street address |
1160 Pine Ave West, McIntyre Bldg Room 710
|
| City |
Montréal |
| State/province |
QC |
| ZIP/Postal code |
H3A 1A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7196 |
Differential gene expression between WT and ERRa-null hearts |
|
| Relations |
| Reanalyzed by |
GSE119085 |
