|
| Status |
Public on Mar 05, 2008 |
| Title |
WT Dendritic Cells_LPS+anti-CD40_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
WT splenic DC ex-vivo from Mice treated with LPS and anti-CD40 agonist
|
| Organism |
Mus musculus |
| Characteristics |
Alymphoplasia mouse Heterozygous littermate.
|
| Treatment protocol |
Mice were treated I.P. with 25 micrograms LPS and 100 micrograms agonistic anti-CD40 antibody clone FGK4.5. 6 hours later splenic DCs were isolated and RNA was isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy
|
| Label |
phycoerythrin
|
| Label protocol |
Double-stranded cDNA is then amplified and labeled using a biotinylated nucleotide analog/ribonucleotide mix in the second IVT reaction.
|
| |
|
| Hybridization protocol |
15 μg of biotinylated cRNA targets were fragmented and hybridized to GeneChip® array during the overnight incubation at 45oC in rotating hybridization oven.
|
| Scan protocol |
After staining the arrays were scanned using Affymetrix GeneChip® Scanner with the laser filter set at 570 nm, pixel size 2.5 μm. The array image data were acquired, and the fluorescent signal intensities were quantified using Affymetrix® MAS 5.0 software with following settings of quantitation parameters: Alpha1=0.05, Alpha2=0.065, Tau=0.015, Gamma1H=0.0045, Gamma1L=0.0045, Gamma2H=0.006, Gamma2L=0.006,
|
| Description |
DCs were CD11c+ bead selected from spleen then flow-sorted to high purity
|
| Data processing |
Raw signal.
|
| |
|
| Submission date |
Mar 07, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Evan F. Lind |
| E-mail(s) |
linde@ohsu.edu
|
| Phone |
503 494-1347
|
| Organization name |
Oregon Health & Science University
|
| Department |
Molecular Microbiology and Immunology
|
| Street address |
Mail Code L220, 3181 SW Sam Jackson Park Road
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7219 |
NIK/NF-kappaB2 regulated gene products. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
