|
| Status |
Public on Feb 20, 2008 |
| Title |
AJ4 Normal Control 24 to 26 weeks |
| Sample type |
RNA |
| |
|
| Source name |
A/J mouse (Jackson Labs) lung tissue
|
| Organism |
Mus musculus |
| Characteristics |
Age matched Control, no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 to 5 microg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MGU74Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from normal mouse lung tissue
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method arbitrarily set to 500
|
| |
|
| Submission date |
Mar 12, 2007 |
| Last update date |
Feb 20, 2008 |
| Contact name |
Robert S Stearman |
| E-mail(s) |
Robert.Stearman@uchsc.edu
|
| Phone |
303-315-2317
|
| Fax |
303-315-5632
|
| Organization name |
UCHSC
|
| Department |
Medicine/Pulmonary
|
| Lab |
Stearman
|
| Street address |
4200 E Ninth Ave
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80262 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (2) |
| GSE7244 |
Expression data from AJ mouse control lung tissue |
| GSE7269 |
AJ mouse lung carcinogenesis study |
|
