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Sample GSM175551 Query DataSets for GSM175551
Status Public on Oct 29, 2007
Title Thoracic aorta age 15mo rep 1
Sample type RNA
 
Source name Rat thoracic aorta at age 15 mo
Organism Rattus norvegicus
Characteristics Fischer 344/Brown Norway F1 hybrid
Thoracic Aorta from male, age 15 months
Extracted molecule total RNA
Extraction protocol The descending thoracic aorta (1 cm) was isolated and excised from 4 rats of each age in randomized order and the tissue was preserved in RNAlater. Tissue was weighed, disrupted by using a bead homogenizer (FastPrep System®; QBIOgene, Carlsbad, CA) and total RNA was purified by using an RNeasy® Fibrous Tissue Mini Kit (Qiagen, Valencia, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on RGU34A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the standard Affymetrix protocol.
Description Gene expression data form thoracic aorta
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
 
Submission date Mar 15, 2007
Last update date Oct 29, 2007
Contact name Steven J Miller
E-mail(s) sjmiller@iupui.edu
Phone 317-274-2657
Fax 317-274-7334
Organization name Indiana University School of Medicine
Department Surgery
Lab Vascular Research
Street address 1001 West 10th Street
City Indianapolis
State/province IN
ZIP/Postal code 46202-2879
Country USA
 
Platform ID GPL85
Series (1)
GSE7281 Development of Progressive Aortic Vasculopathy in a Rat Model of Aging

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 18.9197 A 0.904333
AFFX-MurIL10_at 16.6165 A 0.824672
AFFX-MurIL4_at 7.21857 A 0.993813
AFFX-MurFAS_at 15.6008 A 0.814869
AFFX-BioB-5_at 977.28 P 0.00401721
AFFX-BioB-M_at 2159.63 P 0.00010954
AFFX-BioB-3_at 1248.68 P 0.000509415
AFFX-BioC-5_at 3129.34 P 0.000169227
AFFX-BioC-3_at 2235.78 P 5.16732e-05
AFFX-BioDn-5_at 3425.62 P 0.000509415
AFFX-BioDn-3_at 20980.1 P 5.16732e-05
AFFX-CreX-5_at 42676 P 4.42873e-05
AFFX-CreX-3_at 50939.8 P 4.42873e-05
AFFX-BioB-5_st 60.0009 A 0.544587
AFFX-BioB-M_st 186.012 A 0.368438
AFFX-BioB-3_st 58.1461 A 0.712257
AFFX-BioC-5_st 44.9313 A 0.883887
AFFX-BioC-3_st 16.8507 A 0.949771
AFFX-BioDn-5_st 460.076 A 0.123572
AFFX-BioDn-3_st 506.8 P 0.0151826

Total number of rows: 8799

Table truncated, full table size 284 Kbytes.




Supplementary file Size Download File type/resource
GSM175551.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM175551.EXP.gz 523 b (ftp)(http) EXP
Processed data included within Sample table

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