|
Status |
Public on Jun 04, 2007 |
Title |
embryo_released_at_day3.5_rep1 |
Sample type |
RNA |
|
|
Source name |
Mouse preimplnatation embryos released from the U0126-induced arrest at day 3.5.
|
Organism |
Mus musculus |
Characteristics |
Strain: ICR
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by following the manual of ISOGEN (NIPPON GENE CO., LTD).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 0.1 ug total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
Description |
Gene expression data from embryos released from the U0126-induced arrest at day 3.5.
|
Data processing |
The data were analyzed with GCRMA algorithm
|
|
|
Submission date |
Mar 19, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Eisuke Nishida |
E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
Phone |
+81-75-753-4230
|
Organization name |
Graduate School of Biostudies, Kyoto University
|
Department |
Department of Cell and Developmental Biology
|
Street address |
Kitashirakawa, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8502 |
Country |
Japan |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7309 |
Requirement for ERK MAP kinase in mouse preimplantation development |
|
Relations |
Reanalyzed by |
GSE119085 |