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Sample GSM176491 Query DataSets for GSM176491
Status Public on Jun 04, 2007
Title embryo_released_at_day3.5_rep1
Sample type RNA
 
Source name Mouse preimplnatation embryos released from the U0126-induced arrest at day 3.5.
Organism Mus musculus
Characteristics Strain: ICR
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by following the manual of ISOGEN (NIPPON GENE CO., LTD).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 0.1 ug total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner.
Description Gene expression data from embryos released from the U0126-induced arrest at day 3.5.
Data processing The data were analyzed with GCRMA algorithm
 
Submission date Mar 19, 2007
Last update date Aug 28, 2018
Contact name Eisuke Nishida
E-mail(s) nishida@lif.kyoto-u.ac.jp
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
 
Platform ID GPL1261
Series (1)
GSE7309 Requirement for ERK MAP kinase in mouse preimplantation development
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity

Data table
ID_REF VALUE
1415770_at 41.62735
1415771_at 10165.6
1415772_at 842.4259
1415773_at 2033.6337
1415774_at 117.67529
1415775_at 8777.496
1415776_at 92.34775
1415777_at 6.5369697
1415778_at 2531.33
1415779_s_at 16726.643
1415780_a_at 16.467283
1415781_a_at 19163.48
1415782_at 14047.858
1415783_at 1824.3131
1415784_at 29.904995
1415785_a_at 4616.5225
1415786_at 6.2377534
1415787_at 87.767426
1415788_at 546.92194
1415789_a_at 100.63305

Total number of rows: 45101

Table truncated, full table size 923 Kbytes.




Supplementary file Size Download File type/resource
GSM176491.CEL.gz 3.2 Mb (ftp)(http) CEL

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