|
| Status |
Public on Jun 04, 2007 |
| Title |
embryo_released_at_day4.5_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse preimplnatation embryos released from the U0126-induced arrest at day 4.5.
|
| Organism |
Mus musculus |
| Characteristics |
Strain: ICR
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by following the manual of ISOGEN (NIPPON GENE CO., LTD).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 0.1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from embryos released from the U0126-induced arrest at day 4.5.
|
| Data processing |
The data were analyzed with GCRMA algorithm
|
| |
|
| Submission date |
Mar 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7309 |
Requirement for ERK MAP kinase in mouse preimplantation development |
|
| Relations |
| Reanalyzed by |
GSE119085 |
