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Sample GSM176762 Query DataSets for GSM176762
Status Public on Dec 04, 2007
Title ActCD4, rep1
Sample type RNA
 
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sorting: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: In vitro activation with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD25 hi expressors (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3227.CEL
Data processing RMA
 
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 244.7053463
1415671_at 370.253771
1415672_at 4020.788581
1415673_at 343.4318497
1415674_a_at 407.3900175
1415675_at 270.0314378
1415676_a_at 1840.878015
1415677_at 302.3986743
1415678_at 1157.50367
1415679_at 1814.888581
1415680_at 389.8372653
1415681_at 221.7314378
1415682_at 284.1742949
1415683_at 1247.645723
1415684_at 366.360749
1415685_at 89.54921835
1415686_at 1841.306708
1415687_a_at 1392.734123
1415688_at 2151.84003
1415689_s_at 359.8240994

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176762.CEL.gz 3.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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