NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM176764 Query DataSets for GSM176764
Status Public on Dec 04, 2007
Title ActTreg, rep1
Sample type RNA
 
Source name Treg cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml)for 4 days
Post-culture sort: CD25 hi
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3223.CEL
Data processing RMA
 
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 213.8546791
1415671_at 349.3996869
1415672_at 4571.242005
1415673_at 381.698626
1415674_a_at 381.7008192
1415675_at 344.4728908
1415676_a_at 1577.866854
1415677_at 287.6389021
1415678_at 992.0633731
1415679_at 2071.355647
1415680_at 381.5450586
1415681_at 271.0632599
1415682_at 252.3376395
1415683_at 1443.860009
1415684_at 363.2820774
1415685_at 128.3343495
1415686_at 1699.344448
1415687_a_at 1335.595325
1415688_at 2301.911529
1415689_s_at 494.7338533

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176764.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap