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|
| Status |
Public on Dec 04, 2007 |
| Title |
ActTreg, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Treg cells activated in vitro
|
| Organism |
Mus musculus |
| Characteristics |
Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml)for 4 days
Post-culture sort: CD25 hi
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Description |
jos3223.CEL
|
| Data processing |
RMA
|
| |
|
| Submission date |
Mar 21, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7460 |
Contribution of Foxp3 to the Treg signature |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Data table header descriptions |
| ID_REF |
|
| VALUE |
RMA-unlogged |
| Data table |
| ID_REF |
VALUE |
| 1415670_at |
213.8546791 |
| 1415671_at |
349.3996869 |
| 1415672_at |
4571.242005 |
| 1415673_at |
381.698626 |
| 1415674_a_at |
381.7008192 |
| 1415675_at |
344.4728908 |
| 1415676_a_at |
1577.866854 |
| 1415677_at |
287.6389021 |
| 1415678_at |
992.0633731 |
| 1415679_at |
2071.355647 |
| 1415680_at |
381.5450586 |
| 1415681_at |
271.0632599 |
| 1415682_at |
252.3376395 |
| 1415683_at |
1443.860009 |
| 1415684_at |
363.2820774 |
| 1415685_at |
128.3343495 |
| 1415686_at |
1699.344448 |
| 1415687_a_at |
1335.595325 |
| 1415688_at |
2301.911529 |
| 1415689_s_at |
494.7338533 |
Total number of rows: 45101
Table truncated, full table size 1026 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM176764.CEL.gz |
3.3 Mb |
(ftp)(http) |
CEL |
| Processed data included within Sample table |
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