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Sample GSM176765 Query DataSets for GSM176765
Status Public on Dec 04, 2007
Title ActTregTGF, rep1
Sample type RNA
 
Source name Treg cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD25 hi
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3224.CEL
Data processing RMA
 
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 197.4600092
1415671_at 348.5355243
1415672_at 4336.302866
1415673_at 290.7039691
1415674_a_at 311.7897134
1415675_at 304.6593683
1415676_a_at 1537.602866
1415677_at 243.437775
1415678_at 1043.70179
1415679_at 1779.233076
1415680_at 300.1693633
1415681_at 214.0885806
1415682_at 293.1028664
1415683_at 1140.855122
1415684_at 334.0542318
1415685_at 123.7906812
1415686_at 1819.457216
1415687_a_at 1406.69768
1415688_at 2262.017152
1415689_s_at 431.654353

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176765.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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