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Sample GSM176766 Query DataSets for GSM176766
Status Public on Dec 04, 2007
Title wtActCD4, rep1
Sample type RNA
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3+
Age: 10 day old
Number of mice per chip: 3-6
Tissue: Spleen
Sort: enriched with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD4+, CD25 hi (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3234.CEL
Data processing RMA
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 324.1314378
1415671_at 372.3622329
1415672_at 4627.167407
1415673_at 414.9718068
1415674_a_at 522.2980759
1415675_at 207.9279449
1415676_a_at 2012.517977
1415677_at 242.9131303
1415678_at 910.3147493
1415679_at 1546.345528
1415680_at 478.8678931
1415681_at 279.9292124
1415682_at 347.7457235
1415683_at 1674.558388
1415684_at 428.0600092
1415685_at 157.256538
1415686_at 1798.76225
1415687_a_at 1400.302866
1415688_at 2383.631503
1415689_s_at 331.1944323

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM176766.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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