|
| Status |
Public on Dec 04, 2007 |
| Title |
sfActCD4TGF, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Tconv cells activated in vitro with TGFbeta
|
| Organism |
Mus musculus |
| Characteristics |
Genetic background: BDC2.5/NOD.Foxp3sf
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD4+, CD25 hi expressers (top 45%)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Description |
jos3233.CEL
|
| Data processing |
RMA
|
| |
|
| Submission date |
Mar 21, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7460 |
Contribution of Foxp3 to the Treg signature |
|
| Relations |
| Reanalyzed by |
GSE119085 |
