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Sample GSM176867 Query DataSets for GSM176867
Status Public on Dec 04, 2007
Title sfActCD4TGF, rep1
Sample type RNA
 
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3sf
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD4+, CD25 hi expressers (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3233.CEL
Data processing RMA
 
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 309.3307725
1415671_at 471.6366067
1415672_at 4647.341353
1415673_at 367.5314378
1415674_a_at 590.3842739
1415675_at 284.0071997
1415676_a_at 2094.561002
1415677_at 297.9611758
1415678_at 785.4494254
1415679_at 1655.074295
1415680_at 409.7322096
1415681_at 326.3427346
1415682_at 266.4161362
1415683_at 1933.380383
1415684_at 351.9835658
1415685_at 164.1658581
1415686_at 1849.448319
1415687_a_at 1478.926657
1415688_at 2049.338976
1415689_s_at 314.4441814

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176867.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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