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Sample GSM176868 Query DataSets for GSM176868
Status Public on Dec 04, 2007
Title ActCD8, rep1
Sample type RNA
 
Source name CD8 T cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+,
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3283.CEL
Data processing RMA
 
Submission date Mar 21, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA- unlogged

Data table
ID_REF VALUE
1415670_at 197.3384348
1415671_at 689.4179925
1415672_at 3685.962501
1415673_at 331.0314378
1415674_a_at 255.9627112
1415675_at 348.2811799
1415676_a_at 901.3810044
1415677_at 367.7028664
1415678_at 913.1087147
1415679_at 2632.298505
1415680_at 305.0873934
1415681_at 263.0641493
1415682_at 163.4212977
1415683_at 1090.909352
1415684_at 347.4012098
1415685_at 146.8449901
1415686_at 1035.173957
1415687_a_at 1842.297762
1415688_at 1438.879536
1415689_s_at 613.4296318

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176868.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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