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Sample GSM176881 Query DataSets for GSM176881
Status Public on Dec 04, 2007
Title ActCD8TGF, rep1
Sample type RNA
 
Source name CD8 T cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25 ng/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3284.CEL
Data processing RMA
 
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 181.3917674
1415671_at 681.5385941
1415672_at 3907.832636
1415673_at 284.1273414
1415674_a_at 299.1036401
1415675_at 401.2583153
1415676_a_at 698.3028664
1415677_at 374.9623636
1415678_at 847.1988379
1415679_at 2529.215354
1415680_at 272.2025462
1415681_at 257.2045031
1415682_at 150.0314379
1415683_at 935.5818223
1415684_at 378.6058289
1415685_at 150.4531869
1415686_at 1295.439267
1415687_a_at 1929.592045
1415688_at 1584.27135
1415689_s_at 675.0712057

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176881.CEL.gz 3.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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