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Sample GSM176883 Query DataSets for GSM176883
Status Public on Dec 04, 2007
Title FOXP3rv, rep1
Sample type RNA
Source name Tconv cells retrovirally transduced with FOXP3
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (FOXP3-thy1.1) and cultured for an additional 48hrs.
Post-culture sort: Thy1.1+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3226.CEL
Data processing RMA
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 308.5162703
1415671_at 493.4911619
1415672_at 3782.402866
1415673_at 421.3550212
1415674_a_at 385.5077136
1415675_at 333.337859
1415676_a_at 1928.544854
1415677_at 435.2061879
1415678_at 1334.224088
1415679_at 2474.574456
1415680_at 447.9002399
1415681_at 274.7695529
1415682_at 289.4089654
1415683_at 1494.883814
1415684_at 325.6360118
1415685_at 103.6777138
1415686_at 1702.740531
1415687_a_at 1905.98205
1415688_at 2227.995678
1415689_s_at 456.3723979

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM176883.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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