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Sample GSM176884 Query DataSets for GSM176884
Status Public on Dec 04, 2007
Title CTRLrv, rep1
Sample type RNA
Source name Tconv cells retrovirally transduced with empty vector
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (empty-thy1.1) and cultured for an additional 48hrs.
Post-culture sort: Thy1.1+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3222.CEL
Data processing RMA
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 223.6216136
1415671_at 382.9831184
1415672_at 3754.760061
1415673_at 395.3945998
1415674_a_at 346.3166647
1415675_at 268.1274112
1415676_a_at 1636.146755
1415677_at 344.9128675
1415678_at 1070.860876
1415679_at 2480.592534
1415680_at 373.5451283
1415681_at 269.6922667
1415682_at 239.2255854
1415683_at 1274.617152
1415684_at 367.2844864
1415685_at 122.1956394
1415686_at 1875.434924
1415687_a_at 1231.322412
1415688_at 2301.83173
1415689_s_at 390.7885806

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM176884.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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