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Sample GSM176888 Query DataSets for GSM176888
Status Public on Dec 04, 2007
Title Foxp3rv, rep1
Sample type RNA
 
Source name Tconv cells retroviarally transduced with Foxp3
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (Foxp3-GFP) and cultured for an additional 48hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3241.CEL
Data processing RMA
 
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 185.8407809
1415671_at 548.5232083
1415672_at 3431.347189
1415673_at 303.6147476
1415674_a_at 306.406021
1415675_at 268.5206185
1415676_a_at 1375.11296
1415677_at 324.8502695
1415678_at 1332.521934
1415679_at 2776.786163
1415680_at 291.2346884
1415681_at 296.8540136
1415682_at 203.3843761
1415683_at 1010.367641
1415684_at 294.9124452
1415685_at 112.7907848
1415686_at 1526.942165
1415687_a_at 1808.562926
1415688_at 2381.334962
1415689_s_at 585.6862467

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176888.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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