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Sample GSM176889 Query DataSets for GSM176889
Status Public on Dec 04, 2007
Title Ctrlrv, rep1
Sample type RNA
 
Source name Tconv cells retrovirally transduced with empty vector
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 48hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3240.CEL
Data processing RMA
 
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 212.4758551
1415671_at 560.3930536
1415672_at 3905.044683
1415673_at 334.2403061
1415674_a_at 338.3384666
1415675_at 265.9021749
1415676_a_at 1263.068158
1415677_at 336.6603169
1415678_at 1266.894662
1415679_at 2603.431182
1415680_at 282.0885806
1415681_at 299.9285563
1415682_at 215.6736317
1415683_at 947.9404041
1415684_at 283.7024557
1415685_at 91.56214986
1415686_at 1618.635333
1415687_a_at 1724.70665
1415688_at 2253.10711
1415689_s_at 586.6234911

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176889.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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