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Sample GSM176890 Query DataSets for GSM176890
Status Public on Feb 15, 2008
Title AktTGF, rep1
Sample type RNA
 
Source name Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3455.CEL
Data processing RMA
 
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 158.0037315
1415671_at 564.6319358
1415672_at 4189.178434
1415673_at 402.8258859
1415674_a_at 517.3011878
1415675_at 353.4269023
1415676_a_at 1921.204567
1415677_at 420.3742949
1415678_at 820.8060453
1415679_at 3724.702957
1415680_at 301.9306449
1415681_at 253.7078996
1415682_at 180.6971188
1415683_at 1337.090762
1415684_at 315.7567604
1415685_at 136.074298
1415686_at 1112.674295
1415687_a_at 1833.626862
1415688_at 2034.744401
1415689_s_at 408.0669396

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176890.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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