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Sample GSM176891 Query DataSets for GSM176891
Status Public on Feb 15, 2008
Title GFPTGF, rep1
Sample type RNA
 
Source name Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3459.CEL
Data processing RMA
 
Submission date Mar 22, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 179.3600092
1415671_at 602.7110988
1415672_at 4235.94648
1415673_at 418.8577133
1415674_a_at 540.2710058
1415675_at 353.60391
1415676_a_at 2341.796658
1415677_at 325.6839744
1415678_at 1026.882484
1415679_at 3412.098161
1415680_at 311.4784363
1415681_at 271.17888
1415682_at 217.984607
1415683_at 1279.583043
1415684_at 348.1588185
1415685_at 172.4514458
1415686_at 1156.650913
1415687_a_at 1675.114342
1415688_at 1927.170266
1415689_s_at 450.7093699

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM176891.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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