|
Status |
Public on Mar 23, 2007 |
Title |
p38alpha knock out fetal liver E13.5, biological rep1 |
Sample type |
RNA |
|
|
Source name |
fetal liver with p38alpha knock out
|
Organism |
Mus musculus |
Characteristics |
Genotype: p38alpha KO Age: E13.5
|
Treatment protocol |
No treatment
|
Growth protocol |
No in vitro culture
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Murine Genome U74 Version 2 Set MG-U74A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Gene expression data from fetal liver deficient in p38alpha
|
Data processing |
The data was normalized with RMA (default settings) as implemented in Bioconductor. RMA gives log2 transformed data
|
|
|
Submission date |
Mar 22, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Norbert Schweifer |
E-mail(s) |
nschweifer@gmx.at
|
Organization name |
Boehringer Ingelheim Austria
|
Street address |
Dr. Boehringer Gasse 5-11
|
City |
Vienna |
ZIP/Postal code |
1120 |
Country |
Austria |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7342 |
Expression data from p38 knock out versus wild type fetal liver |
|
Relations |
Reanalyzed by |
GSE119085 |