|
| Status |
Public on Mar 23, 2007 |
| Title |
p38alpha knock out fetal liver E15.5, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
fetal liver with p38alpha knock out
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: p38alpha KO
Age: E15.5
|
| Treatment protocol |
No treatment
|
| Growth protocol |
No in vitro culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Murine Genome U74 Version 2 Set MG-U74A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from fetal liver deficient in p38alpha
|
| Data processing |
The data was normalized with RMA (default settings) as implemented in Bioconductor. RMA gives log2 transformed data
|
| |
|
| Submission date |
Mar 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Norbert Schweifer |
| E-mail(s) |
nschweifer@gmx.at
|
| Organization name |
Boehringer Ingelheim Austria
|
| Street address |
Dr. Boehringer Gasse 5-11
|
| City |
Vienna |
| ZIP/Postal code |
1120 |
| Country |
Austria |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7342 |
Expression data from p38 knock out versus wild type fetal liver |
|
| Relations |
| Reanalyzed by |
GSE119085 |
