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Sample GSM177367 Query DataSets for GSM177367
Status Public on Dec 04, 2007
Title ActCD4, rep2
Sample type RNA
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sorting: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: In vitro activation with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD25 hi expressors (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3243.CEL
Data processing RMA
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 224.9622064
1415671_at 434.3996837
1415672_at 4748.033565
1415673_at 347.3601201
1415674_a_at 405.8259449
1415675_at 206.9101936
1415676_a_at 1521.797892
1415677_at 340.7680796
1415678_at 987.4294673
1415679_at 1791.676365
1415680_at 375.7238267
1415681_at 245.4892862
1415682_at 255.3395309
1415683_at 1118.043889
1415684_at 336.1273993
1415685_at 116.9465645
1415686_at 1629.250121
1415687_a_at 1380.791841
1415688_at 2228.838878
1415689_s_at 352.8541401

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM177367.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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