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Sample GSM177368 Query DataSets for GSM177368
Status Public on Dec 04, 2007
Title ActCD4, rep3
Sample type RNA
 
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sorting: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: In vitro activation with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD25 hi expressors (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3467.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 124.0603181
1415671_at 417.6271464
1415672_at 3417.702866
1415673_at 279.7776437
1415674_a_at 298.2791719
1415675_at 250.9427895
1415676_a_at 1583.25689
1415677_at 226.1162327
1415678_at 991.6341328
1415679_at 2737.52093
1415680_at 290.6495393
1415681_at 284.1650105
1415682_at 208.7190945
1415683_at 1069.275405
1415684_at 213.871529
1415685_at 103.3676454
1415686_at 1141.524017
1415687_a_at 1609.403063
1415688_at 2044.945479
1415689_s_at 409.435852

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177368.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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